Hotspot

psaA-ycf3

LSC · 43260-44099 · 840 bp

Back to Capsicum

Pi 0.00824

MarkerSeek score 57.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 26
pi: 0.00824
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	926-1032 bp	1.0	84.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	908-1014 bp	1.0	84.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	941-1047 bp	1.0	84.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	928-1034 bp	1.0	84.1
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	922-1028 bp	1.0	83.2