Hotspot

trnH-psbA

LSC · 97-564 · 468 bp

Back to Capsicum

Pi 0.00731

MarkerSeek score 49.00

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 17
pi: 0.00731
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	580-595 bp	1.0	79.6
trnH-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	594-609 bp	1.0	79.4
trnH-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	593-608 bp	1.0	79.4
trnH-psbA_p4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	595-610 bp	1.0	79.4
trnH-psbA_p5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	55.0	50.0	58.1	57.6	581-596 bp	1.0	79.1