Hotspot

ycf1

SSC · 123692-129061 · 5370 bp

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Pi 0.00608

MarkerSeek score 51.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5370
variable_sites: 52
pi: 0.00608
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGAGAGATTCCATGCAAA	ATCCCCCTAACCTTTCCACC	50.0	55.0	58.0	58.7	282 bp	1.0	76.1
ycf1_p2	TTCTTCCCCGAGAGATTCCA	ATCCCCCTAACCTTTCCACC	50.0	55.0	57.8	58.7	288 bp	1.0	74.4
ycf1_p3	CCCGAGAGATTCCATGCAAA	CCCCCTAACCTTTCCACCTT	50.0	55.0	58.0	58.9	280 bp	1.0	74.3
ycf1_p4	CCCGAGAGATTCCATGCAAA	CCCCTAACCTTTCCACCTTT	50.0	50.0	58.0	57.0	279 bp	1.0	73.7
ycf1_p5	TTCTTCCCCGAGAGATTCCA	CCCCCTAACCTTTCCACCTT	50.0	55.0	57.8	58.9	286 bp	1.0	72.6