Hotspot

accD

LSC · 56645-58099 · 1455 bp

Back to Capsella

Pi 0.00149

MarkerSeek score 54.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1455
variable_sites: 4
pi: 0.00149
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAATAGAGGCCAGAAGCTCT	TCAGGGTTTTCGTTTAATTCTGA	45.0	34.8	55.9	56.4	1520 bp	1.0	40.2
accD_p2	AAATAGAGGCCAGAAGCTCT	TTCAGGGTTTTCGTTTAATTCTGA	45.0	33.3	55.9	57.1	1521 bp	1.0	40.2
accD_p3	AAATAGAGGCCAGAAGCTCT	CAGGGTTTTCGTTTAATTCTGA	45.0	36.4	55.9	54.9	1519 bp	1.0	40.2
accD_p4	AAATAGAGGCCAGAAGCTCT	TCAGGGTTTTCGTTTAATTCTG	45.0	36.4	55.9	54.9	1520 bp	1.0	40.2
accD_p5	AAATAGAGGCCAGAAGCTCT	TCAGGGTTTTCGTTTAATTCTGAT	45.0	33.3	55.9	56.9	1520 bp	1.0	40.2