Hotspot

rpl32-trnL

SSC · 113382-114109 · 728 bp

Back to Capsella

Pi 0.00984

MarkerSeek score 60.20

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 728
variable_sites: 11
pi: 0.00984
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGAAGGGATATTGGACATCGT	TTTCACCATAGCGGCAACTT	47.6	45.0	57.2	58.1	782-844 bp	1.0	70.1
rpl32-trnL_p2	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	806-868 bp	1.0	69.9
rpl32-trnL_p3	GGAAGGGATATTGGACATCGT	ATTTCACCATAGCGGCAACT	47.6	45.0	57.2	57.9	783-845 bp	1.0	69.9
rpl32-trnL_p4	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	799-861 bp	1.0	69.4
rpl32-trnL_p5	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	804-866 bp	1.0	69.3