Hotspot

psaJ-rpl33

LSC · 66547-66975 · 429 bp

Back to Capsella

Pi 0.0179

MarkerSeek score 57.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 13
pi: 0.0179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGTTCGTTTCGTTAGCAGGT	ATATCTGGAAATTCCCGCGG	50.0	50.0	58.5	57.8	605-606 bp	1.0	76.4
psaJ-rpl33_p2	TGGTTCGTTTCGTTAGCAGG	ATATCTGGAAATTCCCGCGG	50.0	50.0	58.5	57.8	606-607 bp	1.0	76.4
psaJ-rpl33_p3	GGTTCGTTTCGTTAGCAGGT	AATTCCCGCGGATTCTTTCT	50.0	45.0	58.5	57.6	596-597 bp	1.0	74.6
psaJ-rpl33_p4	GGTTCGTTTCGTTAGCAGGT	ATTCCCGCGGATTCTTTCTT	50.0	45.0	58.5	57.6	595-596 bp	1.0	74.6
psaJ-rpl33_p5	TGGTTCGTTTCGTTAGCAGG	ATTCCCGCGGATTCTTTCTT	50.0	45.0	58.5	57.6	596-597 bp	1.0	74.6