Hotspot

atpB-rbcL

LSC · 53694-54492 · 799 bp

Back to Capsella

Pi 0.00543

MarkerSeek score 53.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 7
pi: 0.00543
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	886-887 bp	1.0	72.1
atpB-rbcL_p2	CGAAACCTCTGGATCCGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	885-886 bp	1.0	71.8
atpB-rbcL_p3	ATCGAAACCTCTGGATCCGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	888-889 bp	1.0	70.9
atpB-rbcL_p4	CTACATCCAGTACCGGACCA	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.2	58.0	947-948 bp	1.0	70.8
atpB-rbcL_p5	TACATCCAGTACCGGACCAA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	946-947 bp	1.0	70.7