Hotspot

trnL-trnF

LSC · 46861-47547 · 687 bp

Back to Capsella

Pi 0.0116

MarkerSeek score 52.10

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 12
pi: 0.0116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	777-939 bp	1.0	80.5
trnL-trnF_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	774-936 bp	1.0	77.3
trnL-trnF_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	766-928 bp	1.0	76.7
trnL-trnF_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	739-901 bp	1.0	76.4
trnL-trnF_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	749-911 bp	1.0	76.3