Hotspot

ycf3-trnS

LSC · 43163-44239 · 1077 bp

Back to Capsella

Pi 0.00294

MarkerSeek score 52.30

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 1077
variable_sites: 5
pi: 0.00294
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	CCTTCAGATTGAGCCGACAT	TGTTACGCCTTCAACCACTC	50.0	50.0	57.7	58.1	1127-1129 bp	1.0	76.9
ycf3-trnS_p2	TTCCTTCAGATTGAGCCGAC	TGTTACGCCTTCAACCACTC	50.0	50.0	57.6	58.1	1129-1131 bp	1.0	76.4
ycf3-trnS_p3	TCCTTCAGATTGAGCCGACA	TGTTACGCCTTCAACCACTC	50.0	50.0	58.7	58.1	1128-1130 bp	1.0	73.6
ycf3-trnS_p4	CCTTCAGATTGAGCCGACAT	ACAGTTCCAATGTTACGCCT	50.0	45.0	57.7	57.4	1137-1139 bp	1.0	73.4
ycf3-trnS_p5	CCTTCAGATTGAGCCGACAT	ATGTTACGCCTTCAACCACT	50.0	45.0	57.7	57.4	1128-1130 bp	1.0	73.4