Hotspot

trnT-psbD

LSC · 30949-32205 · 1257 bp

Back to Capsella

Pi 0.00915

MarkerSeek score 52.00

Primer available yes

Label trnT-psbD

Feature Metadata

label_name: trnT-psbD
feature_type: igs
region: LSC
length_bp: 1257
variable_sites: 18
pi: 0.00915
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1408-1411 bp	1.0	80.4
trnT-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1411-1414 bp	1.0	80.2
trnT-psbD_p3	CCAAGACGGCCCTTTTAACT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1430-1433 bp	1.0	80.1
trnT-psbD_p4	ACGGCCCTTTTAACTCAGTG	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1425-1428 bp	1.0	80.0
trnT-psbD_p5	GACGGCCCTTTTAACTCAGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.8	58.0	1426-1429 bp	1.0	79.5