Hotspot

ccsA-ndhD

SSC · 118278-118606 · 329 bp

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Pi 0.0379
MarkerSeek score 66.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
329
variable_sites
28
pi
0.0379
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CATAGTTATGGTTCATTTACATCT AAGGTCGAATCTATTCTATCTAAT 29.2 29.2 52.7 52.6 293-401 bp 1.0 49.9
ccsA-ndhD_p2 CATAGTTATGGTTCATTTACATCT AGGTCGAATCTATTCTATCTAATT 29.2 29.2 52.7 52.6 292-400 bp 1.0 49.9
ccsA-ndhD_p3 CATAGTTATGGTTCATTTACATCT AAGGTCGAATCTATTCTATCTAATT 29.2 28.0 52.7 53.4 293-401 bp 1.0 49.9
ccsA-ndhD_p4 AGTTATGGTTCATTTACATCTAATTGA AAGGTCGAATCTATTCTATCTAAT 25.9 29.2 55.5 52.6 290-398 bp 1.0 49.9
ccsA-ndhD_p5 AGTTATGGTTCATTTACATCTAATTGA AGGTCGAATCTATTCTATCTAATT 25.9 29.2 55.5 52.6 289-397 bp 1.0 49.9