Hotspot

rpl32-trnL-UAG

SSC · 115940-117097 · 1158 bp

Back to Capparis

Pi 0.0305
MarkerSeek score 76.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1158
variable_sites
92
pi
0.0305
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGGCAGCATTGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1249-1322 bp 1.0 88.9
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCATTG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1250-1323 bp 1.0 88.8
rpl32-trnL-UAG_p3 GGGATATTGGGCAGCATTGA AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 1242-1315 bp 1.0 88.4
rpl32-trnL-UAG_p4 GGGATATTGGGCAGCATTGA AACAAAGCGCCATAGCCTAA 50.0 45.0 58.0 57.9 1917-1936 bp 0.833333 74.2
rpl32-trnL-UAG_p5 AGGGATATTGGGCAGCATTG AACAAAGCGCCATAGCCTAA 50.0 45.0 58.0 57.9 1918-1937 bp 0.833333 74.2