Hotspot

ndhF-rpl32

SSC · 115235-115765 · 531 bp

Back to Capparis

Pi 0.024

MarkerSeek score 65.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 531
variable_sites: 29
pi: 0.024
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTGGAATGAAAGGTATCACCCA	TCAATGCTGCCCAATATCCC	45.5	50.0	58.0	58.0	650-1097 bp	1.0	69.4
ndhF-rpl32_p2	GTGGAATGAAAGGTATCACCCA	CAATGCTGCCCAATATCCCT	45.5	50.0	58.0	58.0	649-1096 bp	1.0	69.4
ndhF-rpl32_p3	GTGGAATGAAAGGTATCACCCA	AAAAGCTTTCAATGCTGCCC	45.5	45.0	58.0	57.8	658-1105 bp	1.0	68.0
ndhF-rpl32_p4	AGTGGAATGAAAGGTATCACCC	TCAATGCTGCCCAATATCCC	45.5	50.0	57.8	58.0	651-1098 bp	1.0	67.8
ndhF-rpl32_p5	AGTGGAATGAAAGGTATCACCC	CAATGCTGCCCAATATCCCT	45.5	50.0	57.8	58.0	650-1097 bp	1.0	67.8