Hotspot

rpl16-rps3

LSC · 84021-85297 · 1277 bp

Back to Capparis

Pi 0.0222

MarkerSeek score 66.70

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1277
variable_sites: 69
pi: 0.0222
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TCTTCCTCGATGTTGTTTACGA	TCAAAATTTGGATATTCGTAGACG	40.9	33.3	57.5	55.6	1344-1364 bp	1.0	46.2
rpl16-rps3_p2	TCTTCCTCGATGTTGTTTACGA	CAAAATTTGGATATTCGTAGACG	40.9	34.8	57.5	54.3	1343-1363 bp	1.0	46.2
rpl16-rps3_p3	CTTCCTCGATGTTGTTTACGA	TCAAAATTTGGATATTCGTAGACG	42.9	33.3	56.0	55.6	1343-1363 bp	1.0	46.2
rpl16-rps3_p4	TCTTCCTCGATGTTGTTTACG	TCAAAATTTGGATATTCGTAGACG	42.9	33.3	56.0	55.6	1344-1364 bp	1.0	46.2
rpl16-rps3_p5	TCTTCCTCGATGTTGTTTACGA	AGGAATCAAAATTTGGATATTCGT	40.9	29.2	57.5	55.1	1349-1369 bp	1.0	46.2