Hotspot

rpl36-rps8

LSC · 81951-82444 · 494 bp

Back to Capparis

Pi 0.0256

MarkerSeek score 60.90

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 32
pi: 0.0256
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CGAATGGAAGCCCTTATTTTCA	AAAAGGAATCGGCGGAGAAA	40.9	45.0	57.0	57.8	543-558 bp	1.0	62.1
rpl36-rps8_p2	CGAATGGAAGCCCTTATTTTCA	GACTAAAAGGAATCGGCGGA	40.9	50.0	57.0	57.7	547-562 bp	1.0	61.2
rpl36-rps8_p3	CGAATGGAAGCCCTTATTTTCA	ACTAAAAGGAATCGGCGGAG	40.9	50.0	57.0	57.7	546-561 bp	1.0	61.2
rpl36-rps8_p4	CGAATGGAAGCCCTTATTTTCA	AGGCGCGACTAAAAGGAATC	40.9	50.0	57.0	58.3	553-568 bp	1.0	60.8
rpl36-rps8_p5	CGAATGGAAGCCCTTATTTTCA	GAGGCGCGACTAAAAGGAAT	40.9	50.0	57.0	58.3	554-569 bp	1.0	60.8