Hotspot

petA-psbJ

LSC · 64604-65719 · 1116 bp

Back to Capparis

Pi 0.0239

MarkerSeek score 51.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 65
pi: 0.0239
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1821-1837 bp	1.0	83.8
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1820-1836 bp	1.0	83.8
petA-psbJ_p3	GTTTCAGAGGGCGAATCCAT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1918-1934 bp	1.0	83.6
petA-psbJ_p4	GTTTCAGAGGGCGAATCCAT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1917-1933 bp	1.0	83.6
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1923-1939 bp	1.0	83.5