Hotspot

atpH-atpI

LSC · 14131-15203 · 1073 bp

Back to Capparis

Pi 0.015

MarkerSeek score 61.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 41
pi: 0.015
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.1	58.0	1108-1182 bp	1.0	85.9
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	1166-1240 bp	1.0	85.7
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	1148-1222 bp	1.0	85.6
atpH-atpI_p4	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	1170-1244 bp	1.0	85.0
atpH-atpI_p5	AACGGAAGCAGCAGAAATCA	GCGGGATTCAAGCTCTCATT	45.0	50.0	58.1	58.3	1103-1177 bp	1.0	83.5