Hotspot

trnS-CGA-trnR-UCU

LSC · 9840-10065 · 226 bp

Back to Capparis

Pi 0.0286

MarkerSeek score 63.10

Primer available yes

Label trnS-CGA-trnR-UCU

Feature Metadata

label_name: trnS-CGA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 13
pi: 0.0286
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-CGA-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	301-310 bp	1.0	70.4
trnS-CGA-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	304-313 bp	1.0	70.1
trnS-CGA-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	307-316 bp	1.0	69.6
trnS-CGA-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	310-319 bp	1.0	69.3
trnS-CGA-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	305-314 bp	1.0	67.9