Hotspot

trnK-UUU-rps16

LSC · 4444-5410 · 967 bp

Back to Capparis

Pi 0.0228

MarkerSeek score 60.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 58
pi: 0.0228
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1133-1199 bp	1.0	87.8
trnK-UUU-rps16_p2	TCAGGATCAGTCGTGGTCTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	1129-1195 bp	1.0	87.3
trnK-UUU-rps16_p3	TTCAGGATCAGTCGTGGTCT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	1130-1196 bp	1.0	87.3
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	1271-1337 bp	1.0	87.2
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	1272-1338 bp	1.0	87.2