Hotspot

trnH-GUG-psbA

LSC · 86-468 · 383 bp

Back to Capparis

Pi 0.0439

MarkerSeek score 70.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 37
pi: 0.0439
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ATTTCAGGCTGAGCACAACA	55.0	45.0	58.1	58.0	902-923 bp	1.0	84.3
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TTCAGGCTGAGCACAACATT	55.0	45.0	58.1	58.0	900-921 bp	1.0	84.3
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	TTTCAGGCTGAGCACAACAT	55.0	45.0	58.1	58.0	901-922 bp	1.0	84.3
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	TTCAGGCTGAGCACAACATT	50.0	45.0	57.9	58.0	914-935 bp	1.0	84.2
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	TTTCAGGCTGAGCACAACAT	50.0	45.0	57.9	58.0	915-936 bp	1.0	84.2