Hotspot

rpoC1

LSC · 21078-23941 · 2864 bp

Back to Cannabis

Pi 0.000349

MarkerSeek score 56.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2864
variable_sites: 1
pi: 0.000349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GGGACTCGAATTCACACTGG	GGAAGGACGCTTAATCGGAA	55.0	50.0	58.3	57.7	2958 bp	1.0	75.4
rpoC1_p2	GGGACTCGAATTCACACTGG	GGACGCTTAATCGGAAGGAA	55.0	50.0	58.3	57.7	2954 bp	1.0	75.4
rpoC1_p3	GGGACTCGAATTCACACTGG	AAGGACGCTTAATCGGAAGG	55.0	50.0	58.3	57.7	2956 bp	1.0	75.4
rpoC1_p4	GGGACTCGAATTCACACTGG	AGGAAGGACGCTTAATCGGA	55.0	50.0	58.3	58.5	2959 bp	1.0	73.7
rpoC1_p5	GGGACTCGAATTCACACTGG	AGGACGCTTAATCGGAAGGA	55.0	50.0	58.3	58.5	2955 bp	1.0	73.7