Hotspot

trnS-GCU-trnG

LSC · 8586-9429 · 844 bp

Back to Cannabis

Pi 0.00237

MarkerSeek score 60.20

Primer available yes

Label trnS-GCU-trnG

Feature Metadata

label_name: trnS-GCU-trnG
feature_type: igs
region: LSC
length_bp: 844
variable_sites: 2
pi: 0.00237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG_p1	CAATCCGACGCTTTAGTCCA	ATTCATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	958-959 bp	1.0	76.9
trnS-GCU-trnG_p2	AATCCGACGCTTTAGTCCAC	ATTCATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	957-958 bp	1.0	76.8
trnS-GCU-trnG_p3	ACGGAAAGAGAGGGATTCGA	ATTCATCAAACCGAGGGACC	50.0	50.0	58.2	57.6	1012-1013 bp	1.0	75.7
trnS-GCU-trnG_p4	ACGAAAACGGAAAGAGAGGG	ATTCATCAAACCGAGGGACC	50.0	50.0	57.8	57.6	1018-1019 bp	1.0	75.6
trnS-GCU-trnG_p5	AGCAATCCGACGCTTTAGTC	ATTCATCAAACCGAGGGACC	50.0	50.0	58.4	57.6	960-961 bp	1.0	74.0