Hotspot

atpF

LSC · 13580-14921 · 1342 bp

Back to Canna

Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1342
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 ACTAATTTCGTCGGCTCGAA AAAAAGGGTGAGCGAAGTGA 45.0 45.0 57.4 57.7 1517 bp 1.0 72.1
atpF_p2 ACTAATTTCGTCGGCTCGAA GGGTGAGCGAAGTGATACAA 45.0 50.0 57.4 57.6 1512 bp 1.0 71.8
atpF_p3 ACTAATTTCGTCGGCTCGAA AGGGTGAGCGAAGTGATACA 45.0 50.0 57.4 58.4 1513 bp 1.0 71.3
atpF_p4 ACTAATTTCGTCGGCTCGAA AAAAGGGTGAGCGAAGTGAT 45.0 45.0 57.4 57.4 1516 bp 1.0 70.3
atpF_p5 ACTAATTTCGTCGGCTCGAA AAGGGTGAGCGAAGTGATAC 45.0 50.0 57.4 57.3 1514 bp 1.0 69.5