Hotspot

psaC-ndhE

SSC · 126034-127127 · 1094 bp

Back to Canna

Pi 0
MarkerSeek score 63.50
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
1094
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA CTGAAGCAGCTATTGGGCTA 50.0 50.0 58.1 57.4 1230-1288 bp 1.0 77.7
psaC-ndhE_p2 ACATCCGTCCCAAGGTATCA TGAAGCAGCTATTGGGCTAG 50.0 50.0 58.1 57.4 1229-1287 bp 1.0 77.7
psaC-ndhE_p3 ACATCCGTCCCAAGGTATCA GAAGCAGCTATTGGGCTAGC 50.0 55.0 58.1 58.8 1228-1286 bp 1.0 76.6
psaC-ndhE_p4 CAGGCTCGTACACATTGAGT CTGAAGCAGCTATTGGGCTA 50.0 50.0 57.6 57.4 1190-1248 bp 1.0 75.8
psaC-ndhE_p5 CAGGCTCGTACACATTGAGT TGAAGCAGCTATTGGGCTAG 50.0 50.0 57.6 57.4 1189-1247 bp 1.0 75.8