Hotspot

rpl22-rps19

LSC · 90320-90944 · 625 bp

Back to Canna

Pi 0.0032
MarkerSeek score 60.60
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
625
variable_sites
2
pi
0.0032
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CGCGCTTTGAAAACAGACAT CTGACTTTTGCGAGACATGC 45.0 50.0 58.0 57.8 693-695 bp 1.0 78.0
rpl22-rps19_p2 TTCGCGCTTTGAAAACAGAC CTGACTTTTGCGAGACATGC 45.0 50.0 57.9 57.8 695-697 bp 1.0 77.5
rpl22-rps19_p3 CGCGCTTTGAAAACAGACAT CGAGACATGCGAGAAACGAT 45.0 50.0 58.0 58.4 683-685 bp 1.0 76.3
rpl22-rps19_p4 CGCGCTTTGAAAACAGACAT TTTGCGAGACATGCGAGAAA 45.0 45.0 58.0 58.5 687-689 bp 1.0 76.0
rpl22-rps19_p5 CGCGCTTTGAAAACAGACAT TTTTGCGAGACATGCGAGAA 45.0 45.0 58.0 58.5 688-690 bp 1.0 76.0