Hotspot

atpH-atpI

LSC · 15734-17238 · 1505 bp

Back to Canna

Pi 0.000664
MarkerSeek score 59.10
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1505
variable_sites
1
pi
0.000664
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT ATTTTTGCAACGTTAGCCGC 45.0 45.0 58.0 58.6 1594 bp 1.0 75.1
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT TAGGTGAATCCATGGAGGGT 45.0 50.0 58.0 57.4 1566 bp 1.0 75.0
atpH-atpI_p3 TACCTTCTACAGCTTGGCCT ATTTTTGCAACGTTAGCCGC 50.0 45.0 58.0 58.6 1677 bp 1.0 75.0
atpH-atpI_p4 TACCTTCTACAGCTTGGCCT TAGGTGAATCCATGGAGGGT 50.0 50.0 58.0 57.4 1649 bp 1.0 75.0
atpH-atpI_p5 TACCTTGACCAACTCCAGGT ATTTTTGCAACGTTAGCCGC 50.0 45.0 57.9 58.6 1650 bp 1.0 74.5