Hotspot

rps16-trnQ-UUG

LSC · 6369-7865 · 1497 bp

Back to Canna

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1497
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAACAGAAAAATGGGGCGTG 45.0 50.0 58.1 57.9 1629 bp 1.0 78.4
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTGCCGAACAGAAAAATGGG 45.0 50.0 58.1 57.9 1634 bp 1.0 78.4
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1570 bp 1.0 78.3
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAACAGAAAAATGGGGCGTG 50.0 50.0 58.2 57.9 1640 bp 1.0 77.8
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GTGCCGAACAGAAAAATGGG 50.0 50.0 58.2 57.9 1645 bp 1.0 77.8