Hotspot

ndhD

SSC · 113747-115243 · 1497 bp

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Pi 0

MarkerSeek score 28.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1497
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AATTGGGTCGTTGGGGTTAC	AAACAACTCGAAGCATGGGT	50.0	45.0	58.1	58.0	2389-2415 bp	1.0	79.6
ndhD_p2	CAATTGGGTCGTTGGGGTTA	AAACAACTCGAAGCATGGGT	50.0	45.0	58.1	58.0	2390-2416 bp	1.0	79.6
ndhD_p3	AATTGGGTCGTTGGGGTTAC	GGCATGAAACAACTCGAAGC	50.0	50.0	58.1	58.0	2395-2421 bp	1.0	79.5
ndhD_p4	CAATTGGGTCGTTGGGGTTA	GGCATGAAACAACTCGAAGC	50.0	50.0	58.1	58.0	2396-2422 bp	1.0	79.5
ndhD_p5	AATTGGGTCGTTGGGGTTAC	GAAACAAATTGCCTCTGCCC	50.0	50.0	58.1	57.9	2496-2522 bp	1.0	79.0