Hotspot

ndhG-ndhI

SSC · 117017-117313 · 297 bp

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Pi 0.00169

MarkerSeek score 55.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 297
variable_sites: 1
pi: 0.00169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATCGGTTCAGGCAAATCCAT	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.6	57.9	402-407 bp	1.0	77.1
ndhG-ndhI_p2	ATCGGTTCAGGCAAATCCAT	CAAATTGCTTTGGGCCGTTT	45.0	45.0	57.6	58.4	399-404 bp	1.0	74.6
ndhG-ndhI_p3	ATCGGTTCAGGCAAATCCAT	GCCGTTTACCAGTGTCAGTA	45.0	50.0	57.6	57.6	386-391 bp	1.0	74.4
ndhG-ndhI_p4	ATCGGTTCAGGCAAATCCAT	AATTGCTTTGGGCCGTTTAC	45.0	45.0	57.6	57.6	397-402 bp	1.0	74.3
ndhG-ndhI_p5	TCATGTATCGGTTCAGGCAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	56.9	57.9	408-413 bp	1.0	72.0