Hotspot

rpl32-trnL-UAG

SSC · 111727-112191 · 465 bp

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Pi 0.00681

MarkerSeek score 61.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 465
variable_sites: 6
pi: 0.00681
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCTCAATCGATTTTTACGGGG	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.7	57.9	562-565 bp	1.0	69.6
rpl32-trnL-UAG_p2	GCTCAATCGATTTTTACGGGG	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.7	57.8	555-558 bp	1.0	69.1
rpl32-trnL-UAG_p3	GCTCAATCGATTTTTACGGGG	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.7	57.8	561-564 bp	1.0	69.0
rpl32-trnL-UAG_p4	GCTCAATCGATTTTTACGGGG	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.7	57.8	560-563 bp	1.0	69.0
rpl32-trnL-UAG_p5	GCTCAATCGATTTTTACGGGG	AAGATGTCATGCCGCTACTC	47.6	50.0	57.7	57.8	612-615 bp	1.0	68.6