Hotspot

psaJ-rpl33

LSC · 66937-67429 · 493 bp

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Pi 0.00778

MarkerSeek score 52.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 493
variable_sites: 6
pi: 0.00778
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CATATCTTTCCGTGGCACCA	TGGAAATACCCGTTGATTCCT	50.0	42.9	58.0	57.0	706-707 bp	1.0	64.3
psaJ-rpl33_p2	ACATATCTTTCCGTGGCACC	TGGAAATACCCGTTGATTCCT	50.0	42.9	58.0	57.0	707-708 bp	1.0	64.3
psaJ-rpl33_p3	CATATCTTTCCGTGGCACCA	CTGGAAATACCCGTTGATTCCT	50.0	45.5	58.0	58.1	707-708 bp	1.0	63.5
psaJ-rpl33_p4	ACATATCTTTCCGTGGCACC	CTGGAAATACCCGTTGATTCCT	50.0	45.5	58.0	58.1	708-709 bp	1.0	63.5
psaJ-rpl33_p5	TTGGCGGGTCTCTTGATAGA	TGGAAATACCCGTTGATTCCT	50.0	42.9	58.1	57.0	659-660 bp	1.0	63.5