Hotspot

trnP-UGG-psaJ

LSC · 66334-66801 · 468 bp

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Pi 0.0058

MarkerSeek score 63.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 4
pi: 0.0058
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	527-559 bp	1.0	83.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	526-558 bp	1.0	83.5
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	526-558 bp	1.0	83.5
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	525-557 bp	1.0	83.5
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	538-570 bp	1.0	83.4