Hotspot

trnC-GCA-petN

LSC · 55540-56369 · 830 bp

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Pi 0.00321

MarkerSeek score 51.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 4
pi: 0.00321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	892-907 bp	1.0	79.8
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCAGCCCAAGCAAGATTTA	50.0	45.0	58.1	57.8	888-903 bp	1.0	78.1
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TAAAGCAGCCCAAGCAAGAT	50.0	45.0	58.1	57.8	891-906 bp	1.0	78.1
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	911-926 bp	1.0	77.6
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GCAGCCCAAGCAAGATTTAC	50.0	50.0	58.1	57.7	887-902 bp	1.0	77.6