Hotspot

atpF-atpH

LSC · 40611-41079 · 469 bp

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Pi 0.0114

MarkerSeek score 67.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 9
pi: 0.0114
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TAAATCCGAAACTTCCGGCG	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.6	58.0	635-694 bp	1.0	71.9
atpF-atpH_p2	GCAAGTAGCCCAAGCAAAGA	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.8	58.0	611-670 bp	1.0	71.0
atpF-atpH_p3	TAAATCCGAAACTTCCGGCG	AGCAGAGGGAAAAATACGAGG	50.0	47.6	58.6	57.7	636-695 bp	1.0	69.8
atpF-atpH_p4	GCAAGTAGCCCAAGCAAAGA	AGCAGAGGGAAAAATACGAGG	50.0	47.6	58.8	57.7	612-671 bp	1.0	69.0
atpF-atpH_p5	TAAATCCGAAACTTCCGGCG	GCAGAGGGAAAAATACGAGG	50.0	50.0	58.6	56.2	635-694 bp	1.0	65.7