Hotspot

psbM-trnQ-UUG

LSC · 33227-34206 · 980 bp

Back to Canavalia

Pi 0.00239

MarkerSeek score 61.80

Primer available yes

Label psbM-trnQ-UUG

Feature Metadata

label_name: psbM-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 4
pi: 0.00239
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnQ-UUG_p1	TCATTCTAGTTCCGACTGCC	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.0	58.7	1088-1099 bp	1.0	68.2
psbM-trnQ-UUG_p2	CATTCTAGTTCCGACTGCCT	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.0	58.7	1087-1098 bp	1.0	68.1
psbM-trnQ-UUG_p3	TCATTCTAGTTCCGACTGCC	TGTCAAGTCGAAATTGGGGC	50.0	50.0	57.0	58.8	1120-1131 bp	1.0	67.5
psbM-trnQ-UUG_p4	CATTCTAGTTCCGACTGCCT	TGTCAAGTCGAAATTGGGGC	50.0	50.0	57.0	58.8	1119-1130 bp	1.0	67.5
psbM-trnQ-UUG_p5	TCATTCTAGTTCCGACTGCC	GGGTTTTGGTCCCGCTATTC	50.0	55.0	57.0	58.9	1079-1090 bp	1.0	66.4