Hotspot

psbD-trnT-GGU

LSC · 30032-31464 · 1433 bp

Back to Canavalia

Pi 0.00093

MarkerSeek score 64.10

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1433
variable_sites: 2
pi: 0.00093
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CACAAAAACGAAACGGTCCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1335-1587 bp	1.0	82.2
psbD-trnT-GGU_p2	CCACAAAAACGAAACGGTCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1336-1588 bp	1.0	82.2
psbD-trnT-GGU_p3	CCCACAAAAACGAAACGGTC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1337-1589 bp	1.0	82.2
psbD-trnT-GGU_p4	CACAAAAACGAAACGGTCCC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.2	58.1	1338-1590 bp	1.0	82.1
psbD-trnT-GGU_p5	CCACAAAAACGAAACGGTCC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.2	58.1	1339-1591 bp	1.0	82.1