Hotspot

trnG-GCC-psbZ

LSC · 26080-26699 · 620 bp

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Pi 0.00565

MarkerSeek score 61.80

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 4
pi: 0.00565
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGATGGTTGGTCG	50.0	55.0	57.8	58.0	727-774 bp	1.0	82.9
trnG-GCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	TTGCTTCTCCTGATGGTTGG	50.0	50.0	57.8	57.8	730-777 bp	1.0	81.4
trnG-GCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TCCTGATGGTTGGTCGAGTA	50.0	50.0	57.8	57.8	723-770 bp	1.0	81.3
trnG-GCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	GCTTCTCCTGATGGTTGGTC	50.0	55.0	57.8	58.3	728-775 bp	1.0	81.0
trnG-GCC-psbZ_p5	CGTCTTCTCCTTGGCAAAGA	TTCTCCTGATGGTTGGTCGA	50.0	50.0	57.8	58.4	726-773 bp	1.0	80.2