Hotspot

ycf3-psaA

LSC · 19966-20702 · 737 bp

Back to Canavalia

Pi 0.0023

MarkerSeek score 60.00

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 3
pi: 0.0023
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	GAGAAATGACCGGGTTTTGC	50.0	50.0	58.0	57.7	890-902 bp	1.0	79.0
ycf3-psaA_p2	AGCTATAGCCCTTACCCCTG	GAGAAATGACCGGGTTTTGC	55.0	50.0	58.0	57.7	896-908 bp	1.0	78.7
ycf3-psaA_p3	TTGGTTGAAGATCACAGGGC	GAGAAATGACCGGGTTTTGC	50.0	50.0	58.1	57.7	854-866 bp	1.0	78.4
ycf3-psaA_p4	TGGTTTAATCAAGCCGCTGA	GAGAAATGACCGGGTTTTGC	45.0	50.0	57.8	57.7	928-940 bp	1.0	77.4
ycf3-psaA_p5	AGATCACAGGGCGTTTTGAA	GAGAAATGACCGGGTTTTGC	45.0	50.0	57.7	57.7	846-858 bp	1.0	76.9