Hotspot

trnK-UUU-rbcL

LSC · 5398-6407 · 1010 bp

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Pi 0.00546

MarkerSeek score 64.80

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 7
pi: 0.00546
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGTCGCTCTTAAAAGCCGAG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.0	58.0	1107-1188 bp	1.0	82.2
trnK-UUU-rbcL_p2	AGTCGCTCTTAAAAGCCGAG	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.0	58.0	1122-1203 bp	1.0	82.1
trnK-UUU-rbcL_p3	GAGTCGCTCTTAAAAGCCGA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.0	58.0	1108-1189 bp	1.0	82.1
trnK-UUU-rbcL_p4	GAGTCGCTCTTAAAAGCCGA	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.0	58.0	1123-1204 bp	1.0	82.1
trnK-UUU-rbcL_p5	TGGAGTCGCTCTTAAAAGCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.9	58.0	1110-1191 bp	1.0	81.4