Pi
0.0453
MarkerSeek score
70.80
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | ATTTCAAGTTGCCGAGTGGT | AACCAAACCCACGGATTTGA | 45.0 | 45.0 | 58.0 | 57.9 | 2031 bp | 0.333333 | 52.6 |
| ycf1_p2 | CTCTGGGAGCAGTTTCCATC | AACCAAACCCACGGATTTGA | 55.0 | 45.0 | 58.0 | 57.9 | 2150 bp | 0.333333 | 52.5 |
| ycf1_p3 | AGCTAAAAATATCGCCCGCA | AACCAAACCCACGGATTTGA | 45.0 | 45.0 | 58.0 | 57.9 | 2058 bp | 0.333333 | 52.4 |
| ycf1_p4 | ATTTCAAGTTGCCGAGTGGT | GAAGAACCAAACCCACGGAT | 45.0 | 50.0 | 58.0 | 58.1 | 2035 bp | 0.333333 | 52.4 |
| ycf1_p5 | CTCTGGGAGCAGTTTCCATC | GAAGAACCAAACCCACGGAT | 55.0 | 50.0 | 58.0 | 58.1 | 2154 bp | 0.333333 | 52.3 |
| ycf1_p1 | CCATACTGAAACGACTGCCA | AAAAGTATGCGGGATCGGAC | 50.0 | 50.0 | 57.9 | 58.1 | 735-807 bp | 1.0 | 90.4 |
| ycf1_p2 | ACCATACTGAAACGACTGCC | AAAAGTATGCGGGATCGGAC | 50.0 | 50.0 | 57.9 | 58.1 | 736-808 bp | 1.0 | 90.4 |
| ycf1_p3 | CCATACTGAAACGACTGCCA | TCAAATCCGTGGGTTTGGTT | 50.0 | 45.0 | 57.9 | 57.9 | 1042-1096 bp | 1.0 | 88.9 |
| ycf1_p4 | ACCATACTGAAACGACTGCC | TCAAATCCGTGGGTTTGGTT | 50.0 | 45.0 | 57.9 | 57.9 | 1043-1097 bp | 1.0 | 88.9 |
| ycf1_p5 | CCATACTGAAACGACTGCCA | ATCCGTGGGTTTGGTTCTTC | 50.0 | 50.0 | 57.9 | 58.1 | 1038-1092 bp | 1.0 | 88.8 |