Hotspot

trnR-UCU-atpA

LSC · 11013-11198 · 186 bp

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Pi 0.0352

MarkerSeek score 73.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 9
pi: 0.0352
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATTCGGAATGAAAGCGTCCA	AAGACATTCACCGAGGAAGC	45.0	50.0	57.9	57.9	311-358 bp	1.0	84.4
trnR-UCU-atpA_p2	TCGGAATGAAAGCGTCCATT	AAGACATTCACCGAGGAAGC	45.0	50.0	57.9	57.9	309-356 bp	1.0	84.4
trnR-UCU-atpA_p3	TTCGGAATGAAAGCGTCCAT	AAGACATTCACCGAGGAAGC	45.0	50.0	57.9	57.9	310-357 bp	1.0	84.4
trnR-UCU-atpA_p4	CGGAATGAAAGCGTCCATTG	AAGACATTCACCGAGGAAGC	50.0	50.0	57.8	57.9	308-355 bp	1.0	83.9
trnR-UCU-atpA_p5	TCGGAATGAAAGCGTCCATT	TCAGGAACAATTGGAACGCT	45.0	45.0	57.9	57.7	265-312 bp	1.0	83.4