Hotspot

trnK-UUU-rps16

LSC · 4626-5713 · 1088 bp

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Pi 0.0159
MarkerSeek score 68.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1088
variable_sites
24
pi
0.0159
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCGATGGTATGGACGAATC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1261-1288 bp 1.0 81.7
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1260-1287 bp 1.0 81.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1199-1226 bp 1.0 81.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1198-1225 bp 1.0 81.2
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 1208-1235 bp 1.0 80.2