Hotspot

rpl32-trnL-UAG

SSC · 111605-112187 · 583 bp

Back to Campylotropis

Pi 0.0119
MarkerSeek score 70.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
583
variable_sites
44
pi
0.0119
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACGGGGAATTCAAAAAGCTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 56.1 57.9 561-671 bp 1.0 73.4
rpl32-trnL-UAG_p2 ACGGGGAATTCAAAAAGCTT AGCAGCGTGTCTACCAATTT 40.0 45.0 56.1 57.8 554-664 bp 1.0 73.0
rpl32-trnL-UAG_p3 ACGGGGAATTCAAAAAGCTT CCTAAGAGCAGCGTGTCTAC 40.0 55.0 56.1 57.8 560-670 bp 1.0 72.8
rpl32-trnL-UAG_p4 ACGGGGAATTCAAAAAGCTT CTAAGAGCAGCGTGTCTACC 40.0 55.0 56.1 57.8 559-669 bp 1.0 72.8
rpl32-trnL-UAG_p5 ACGGGGAATTCAAAAAGCTT GAGCAGCGTGTCTACCAATT 40.0 50.0 56.1 58.3 555-665 bp 1.0 72.3