Hotspot

accD-psaI

LSC · 57934-58265 · 332 bp

Back to Campylotropis

Pi 0.0246

MarkerSeek score 71.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 332
variable_sites: 32
pi: 0.0246
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	448-493 bp	0.9	85.7
accD-psaI_p2	TTGATTCAATCGTACCGCGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	454-499 bp	0.9	85.0
accD-psaI_p3	TTGATTCAATCGTACCGCGT	TGCCGGAAATACTAGACCCA	45.0	50.0	58.0	58.1	438-483 bp	0.9	85.0
accD-psaI_p4	TTGATTCAATCGTACCGCGT	GCCGGAAATACTAGACCCAC	45.0	55.0	58.0	57.8	437-482 bp	0.9	84.2
accD-psaI_p5	TGATTCAATCGTACCGCGTA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.2	58.0	447-492 bp	0.9	78.9