Hotspot

trnQ-UUG-rps16

LSC · 54378-54791 · 414 bp

Back to Campylotropis

Pi 0.013

MarkerSeek score 66.80

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 25
pi: 0.013
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	55.0	50.0	58.1	58.0	493-514 bp	1.0	85.6
trnQ-UUG-rps16_p2	GTCAAATCGAAAATGGGGCG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.1	58.0	554-575 bp	1.0	84.8
trnQ-UUG-rps16_p3	GAGGTTCGAATCCTTCCGTC	ATGTCTTTCAAGTCGCACGT	55.0	45.0	58.1	58.1	495-516 bp	1.0	84.6
trnQ-UUG-rps16_p4	GAGGTTCGAATCCTTCCGTC	GCACGTTGCTTTTTACCACA	55.0	45.0	58.1	57.8	481-502 bp	1.0	84.2
trnQ-UUG-rps16_p5	GTCAAATCGAAAATGGGGCG	ATGTCTTTCAAGTCGCACGT	50.0	45.0	58.1	58.1	556-577 bp	1.0	83.8