Hotspot

psbM-petN

LSC · 31688-32460 · 773 bp

Back to Campylotropis

Pi 0.0149

MarkerSeek score 73.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 51
pi: 0.0149
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTTATTCT	TTTTTCCCTTTCCCTCGTGG	38.1	50.0	55.2	58.0	874-911 bp	1.0	57.7
psbM-petN_p2	TGCTACTGCACTGTTTATTCT	CGTGGTATGGGGAAGAAGTG	38.1	55.0	55.2	58.0	859-896 bp	1.0	57.7
psbM-petN_p3	TGCTACTGCACTGTTTATTCT	TCTTGCTTGGGCTGCTTTAA	38.1	45.0	55.2	58.0	907-944 bp	1.0	57.5
psbM-petN_p4	TGCTACTGCACTGTTTATTCT	TTCCCTTTCCCTCGTGGTAT	38.1	50.0	55.2	58.0	871-908 bp	1.0	57.5
psbM-petN_p5	TGCTACTGCACTGTTTATTCT	TCGTGGTATGGGGAAGAAGT	38.1	50.0	55.2	58.1	860-897 bp	1.0	57.5