Hotspot

trnT-GGU-trnD-GUC

LSC · 30185-30449 · 265 bp

Back to Campylotropis

Pi 0.0202

MarkerSeek score 75.50

Primer available yes

Label trnT-GGU-trnD-GUC

Feature Metadata

label_name: trnT-GGU-trnD-GUC
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 23
pi: 0.0202
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnD-GUC_p1	GAACCGATGACTTACGCCTT	CGCTTTTCCCTGGGATTGTA	50.0	50.0	58.0	57.9	393-430 bp	1.0	87.9
trnT-GGU-trnD-GUC_p2	CCATGGCGTTACTCTACCAC	CGCTTTTCCCTGGGATTGTA	55.0	50.0	58.1	57.9	372-409 bp	1.0	87.7
trnT-GGU-trnD-GUC_p3	TGGCGTTACTCTACCACTGA	CGCTTTTCCCTGGGATTGTA	50.0	50.0	58.1	57.9	369-406 bp	1.0	87.6
trnT-GGU-trnD-GUC_p4	CCGATGACTTACGCCTTACC	CGCTTTTCCCTGGGATTGTA	55.0	50.0	58.2	57.9	390-427 bp	1.0	86.8
trnT-GGU-trnD-GUC_p5	TTGAACCGATGACTTACGCC	CGCTTTTCCCTGGGATTGTA	50.0	50.0	58.3	57.9	395-432 bp	1.0	85.7