Hotspot

ycf3-psaA

LSC · 18806-19479 · 674 bp

Back to Campylotropis

Pi 0.0116

MarkerSeek score 68.90

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 50
pi: 0.0116
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AAGTTGCGGAGTCTTGGTTT	TTTCACTTCTGGTTCCGGTG	45.0	50.0	57.9	58.0	826-857 bp	1.0	88.0
ycf3-psaA_p2	TGGTTTAATCAAGCCGCTGA	TTTCACTTCTGGTTCCGGTG	45.0	50.0	57.8	58.0	812-843 bp	1.0	87.0
ycf3-psaA_p3	AAGCTATAGCCCTTACCCCC	TTTCACTTCTGGTTCCGGTG	55.0	50.0	58.3	58.0	781-812 bp	1.0	86.6
ycf3-psaA_p4	GAAGTTGCGGAGTCTTGGTT	TTTCACTTCTGGTTCCGGTG	50.0	50.0	58.4	58.0	827-858 bp	1.0	85.2
ycf3-psaA_p5	CCCTTACCCCCGGGAATTAT	TTTCACTTCTGGTTCCGGTG	55.0	50.0	58.5	58.0	772-803 bp	1.0	84.4