Hotspot

trnH-GUG-psbA

LSC · 94-382 · 289 bp

Back to Campylotropis

Pi 0.0154

MarkerSeek score 68.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 25
pi: 0.0154
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCCTCTAGATCTAGCTGCGA	50.0	55.0	57.9	57.8	355-376 bp	1.0	86.7
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TCCCTCTAGATCTAGCTGCG	50.0	55.0	57.9	57.8	356-377 bp	1.0	86.7
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	CCCTCTAGATCTAGCTGCGA	50.0	55.0	58.2	57.8	356-377 bp	1.0	86.4
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TCCCTCTAGATCTAGCTGCG	50.0	55.0	58.2	57.8	357-378 bp	1.0	86.4
trnH-GUG-psbA_p5	GCGAATGACGGGAATTGAAC	CCCTCTAGATCTAGCTGCGA	50.0	55.0	57.8	57.8	400-421 bp	1.0	85.5