Hotspot

rpl19

Genome · 28872-29225 · 354 bp

Back to Campylaephora

Pi 0.0932

MarkerSeek score 72.90

Primer available yes

Label rpl19

Feature Metadata

label_name: rpl19
feature_type: gene
region: Genome
length_bp: 354
variable_sites: 33
pi: 0.0932
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl19_p1	CTACAGCGACAAAGCCAGAA	AGTGGTGATGCAAGCTTTCA	50.0	45.0	58.2	58.0	1478-1483 bp	1.0	85.1
rpl19_p2	CTACAGCGACAAAGCCAGAA	TCAGTGGTGATGCAAGCTTT	50.0	45.0	58.2	58.0	1480-1485 bp	1.0	85.1
rpl19_p3	TCAGCATTATCGCCAAGCAT	AGTGGTGATGCAAGCTTTCA	45.0	45.0	58.0	58.0	1694 bp	0.5	59.7
rpl19_p4	TCAGCATTATCGCCAAGCAT	TCAGTGGTGATGCAAGCTTT	45.0	45.0	58.0	58.0	1696 bp	0.5	59.7
rpl19_p5	TCAGCATTATCGCCAAGCAT	TGGTTGCACCAGGAAAAAGT	45.0	45.0	58.0	58.1	1369 bp	0.5	58.6